Table 1: Cryopreservation of autologous peripheral blood stem cell grafts in cancer patients; a summary of the effects on immunocompetent cells when grafts were prepared with 2%, 4%, 5%, and 10% DMSO.

T cell populationFunctional characteristicsEffects of cryopreservation with DMSO at various concentrations

Major T cell subsets
 CD3+CD4+T helperNo effect of different DMSO levels
 CD3+CD8+CytotoxicNo effect of different DMSO levels
 CD3+CD56+CD16+NK T cellsHighest viability with 2% DMSO
 CD3+CD56+CD16-NK T cellsHighest viability with 2% DMSO

CD62L-defined subsets
 CD4+CD62+, CD8+CD62+Naive and central memoryHighest viability when using DMSO 5%
 CD4+CD6 2 l o w , CD8+CD6 2 l o w Late effector T cellsCD4+ cells show highest viability with DMSO 2% and 4%
 CD4+CD62-, CD8+CD62-Effector memoryNo effect of different DMSO levels

Subsets defined by chemokine receptor expression
 CD4+CCR7+, CD8+CCR7+Naive and central memory T cellsThe CD4+ cells showed decreased viability when using 10% DMSO
 CD3+CD45+CCR4-CCR6-Decreased viability when using 2% DMSO
 Other CCR2, CCR4, CCR7No effect of different DMSO levels
 defined T cell subsets
 CD4+CD25+FoxP3+ T cellsNatural regulatory T cellsDecreased viability when using 10% DMSO

Autografts were prepared for cancer patients after mobilisation with chemotherapy plus G-CSF. After the aphereses cell concentrations were adjusted and cells stored in nitrogen for 5-6 years as described in the text [50].