Phenotypic Transition of the Collecting Duct Epithelium in Congenital Urinary Tract Obstruction
Figure 7
Gene expression in normal and obstructed fetal kidneys. (a) Quantitative PCR analysis of total RNA extracted from 10, 15, and 18 weeks gestation normal kidneys, for genes encoding epithelial-specific (E-cad, N-cad, -cat, and -cat) and mesenchymal-specific (vimentin, ColIVa3, ColIVa5 and -SMA) proteins. Values are expressed as fold change in mRNA expression compared to the normal 10 weeks gestation kidney as reference. (b) Quantitative PCR analysis of total RNA extracted from obstructed kidneys at 18 weeks gestation (mild and severe) and 36 weeks gestation (severe), and from 18 weeks normal kidney. Values are expressed as fold change in mRNA expression compared to the normal 18 weeks gestation kidney as reference.
