DCs were transduced with nothing (No treated), CD80 lenti, CD86 lenti, NC lenti, or both CD80 lenti and CD86 lenti. (a) After 6 days of culture, the cells were then incubated with LPS (1 μg/mL) for 18 hours. Expression of CD80 and CD86 was determined by flow cytometry. The data show the effect of recombinant lentivirus on inhibition of target molecule expression. Values expressed as mean 1 SD from three independent experiments; * compared to NC lenti/No treated; ** compared to CD80 lenti; *** compared to CD86 lenti. (b) C3H BM-derived DCs were cultured over 6 days. DCs were stimulated or unstimulated with LPS (1 μg/mL) for 18 hours. Upregulation of CD80 and CD86 in response to LPS was also suppressed by CD80 lenti and CD86 lenti. The mean fluorescence intensity (MFI) of GFP+ cells expressing the marker of interest is indicated.
