Methodology Report

Nonviral Gene Delivery to Mesenchymal Stem Cells Using Cationic Liposomes for Gene and Cell Therapy

Figure 2

Bright field (a) and fluorescence (b) microscopic images of lipofectamine-mediated pDNA-GFP transfer in MSC, 24 hours after transfection. Images obtained by fluorescence optical microscopy (100x). Flow cytometry profiles of nontransfected hMSC (white) and hMSC transfected with pDNA-GFP using Lipofectamine2000 L / D = 1 . 2 5 (grey) and respective statistical analysis (c).
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(a)
735349.fig.002b
(b)
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(c)