Nonviral Gene Delivery to Mesenchymal Stem Cells Using Cationic Liposomes for Gene and Cell Therapy
Figure 5
(a) CFU assay of MSC after transfection (in black). Two different controls were assayed: cells which have not been in contact with lipofectamine and pDNA (white), and cells which have only been in contact with lipofectamine ( L—grey). MSC were transfected at passage 4 with a transfection efficiency of 20%. Results are presented as of two independent experiments with duplicates. (b) Oil red-O staining and (c) Alkaline Phosphatase and Von Kossa stainings were tested in order to analyse the maintenance of adipogenic and osteogenic differentiation potential after transfection, respectively. The assay was performed in duplicate, and images were obtained through fluorescence optical microscopy (200x).
