A New MAP Kinase Protein Involved in Estradiol-Stimulated Reproduction of the Helminth Parasite Taenia crassiceps
Figure 4
Specific detection of ERK-like in Taenia crassiceps. FACS assays showed that ERK-like detected in T. crassiceps was not a contamination product from host immune cells CD3, CD4, CD8, and CD19. As expected, ERK-like was specifically detected in paramyosin positive cells, a cytoskeleton protein only present in cestodes and some insects (a). By using the Mean of Fluorescence Intensity (MFI), no differences in total ERK-like and pERK were observed between control and 17-estradiol cysticerci (b). Control Parasites treated with the vehicle where hormone and inhibitors were dissolved. Red unstained cells; green=control parasites; blue -treated parasites; brown ERK inhibitor-treated parasites; SSC Side Scatter; FSC Forward Scatter; pERK ERK phosphorylated form; MFI Fluorescence average.
