Table 2: T. cruzi genes selected by in silico analysis and predicted properties of the encoded hypothetical proteins.

T. cruzi GeneDB Gene ID No.OrthologsProbability TM ( d ) GPI ( e ) Conserved domains (and E-value ) ( f )
L. major and T. brucei Gene ID No. ( a ) SPP ( b ) CSP ( c )

Tc00.1047053506417.30LmjF22.0225Tb927.8.2180 0.937 0.9171YesNone

Tc00.1047053506155.99LmjF36.5220Tb11.01.24700.9840.9620NoGlucosidase II beta subunit-like (E = 1.6 e-13)

Tc00.1047053506467.29LmjF26.2000Tb09.160.10700.8110.7710YesMethyltransferase domain (E = 4.3 e-4)

Tc00.1047053511901.30LmjF24.2160Tb927.8.60800.9890.8980NoGlycerophosphoryl diester phosphodiesterase (E = 5.7 e-8)

Tc00.1047053511871.30LmjF25.1010 Tb927.3.950 0.9790.9580No2OG-Fe(II) oxygenase (E = 1.7 e-13)

Tc00.1047053505789.10 ( g ) LmjF19.0540 Tb927.8.6700 ( h ) 1.0000.7685NoLipocalin signature (E = 0.0)

LmjF19.0570 Tb11.39.0005 ( i )
Tc00.1047053509669.70LmjF29.1600Tb927.3.41900.9990.9809NoEndomembrane protein 70 (E = 0.0)

Tc00.1047053507765.20LmjF11.0720Tb11.02.44000.9930.9860NoNone

Tc00.1047053510101.470LmjF11.0720Tb11.02.44000.9310.9190NoNone

Tc00.1047053510443.30LmjF30.3150Tb927.6.45000.9030.8381NoTranslocon-associated protein beta (TRAPB) (E = 4.1 e-4)

Tc00.1047053509799.50LmjF36.5570Tb10.6k15.11300.9810.9311NoNone

Tc00.1047053509835.30 ( g ) LmjF19.0540 Tb927.8.6700 ( h ) 0.8660.8035NoHeavy-metal-associated domain, Heavy metaltransport/detoxification (E = 0.0)

LmjF19.0570 Tb11.39.0005 ( i )
Tc00.1047053509999.10LmjF29.1200 Tb927.3.38201.0000.9523NoNone

( a ) L. major and T. brucei gene ID No. of putative orthologs from GeneDB.
Nonsyntenic predicted orthologs in the TriTrypDB server.
( b ) SPP Signal peptide probability predicted by SignalP 3.0.
( c ) CSP Maximal cleavage site probability predicted by SignalP 3.0.
( d ) TM Number of transmembrane domains (other than peptide signal sequence) predicted by TMHMM 2.0.
( e ) GPI Identification of GPI-anchor signal by GPI-SOM.
( f ) Conserved domains (and corresponding E-value) from InterPro, PROSITE or Pfam.
( g ) Tc00.1047053505789.10 and Tc00.1047053509835.30 are homologous genes presenting 45% identity at the protein level. These genes are members of a multigene family including Tc00.1047053505789.20, Tc00.1047053509441.10, Tc00.1047053510063.30, Tc00.1047053510065.10, and Tc00.1047053504235.9.
( h ) Tb927.8.6700, Tb927.8.6710, Tb927.8.6720, and Tb927.8.6730 represent the paralogs/orthologs of Tc00.1047053505789.10, Tc00.1047053505789.20, Tc00.1047053509441.10, Tc00.1047053510063.30, and Tc00.1047053510065.10 in the TriTrypDB server.
( i ) Tb11.39.0005 represents the predicted ortholog of Tc00.1047053509835.30 and Tc00.1047053504235.9 in the TriTrypDB server.