Reduction of Foxp𝟑⁺ Cells by Depletion with the PC61 mAb Induces Mortality in Resistant BALB/c Mice Infected with Toxoplasma gondii

Figure 2

Kinetics analysis of the depletion of CD4⁺CD25⁺ cells. BALB/c mice were injected ip with 100 𝜇g ((a)–(e)) or 200 𝜇g ((f)–(j)) of PC61 mAb. Spleen cells were obtained at different time points and immunofluorescence was carried out as described in Figure 1 using anti-CD4-FITC and anti-CD25-APC mAbs. The lymphocyte region was first identified and gated by FSC and SSC characteristics, the CD4⁺ region was subgated and 10,000 CD4⁺ events were captured. Numbers indicate % of CD4⁺CD25⁺ cells within the CD4⁺ population.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)