Functional Differences between the N-Terminal Domains of Mouse and Human Myosin Binding Protein-C

Figure 2

Effects of mC0C1 and hC0C1 on steady state force and rate of tension redevelopment (𝑘_tr) in permeabilized rat trabeculae. (a) Preincubation of trabeculae with 80 𝜇M mC0C1 or hC0C1 did not affect resting force at pCa 9.0 or maximal Ca²⁺ activated force at pCa 4.5. However, hC0C1 increased force (b) and 𝑘_tr (c) at intermediate [Ca²⁺] (pCa 5.3) with effects significant at 50 𝜇M. mC0C1 increased force at 50 𝜇M but did not significantly increase 𝑘_tr. Data are mean ± SEM (𝑛 = 4 for hC0C1 and 𝑛 = 3 for mC0C1). Asterisks denote significant differences in compared values obtained prior to protein addition (𝑃<.05).

(a)

(b)

(c)