Regulation of Adrenal Aldosterone Production by Serine Protease Prostasin
Figure 2
Effect of adenovirus-mediated overexpression of prostasin on CYP11B2 expression (a) and aldosterone production (b) in H295R cells. Cells were infected with 6 105 pfu of control virus (Ad-LacZ) or adenovirus carrying human prostasin (Ad-hPRSS8) for 1 hour. Forty eight hours after infection, total RNA was isolated for the real time PCR and the medium was collected for the aldosterone measurement. (c) Effect of PAR-1 or PAR-2 knock down on prostasin-induced CYP11B2 expression in H295R cells. Cells were transfected with siRNA for either PAR-1 or PAR-2 for 48 hours and then treated with prostasin for another 24 hours. mRNA expression of CYP11B2 was determined by the real time PCR. Values are means SD . versus Ad-LacZ (a), (b), or vehicle (c).
