Epidermal Growth Factor (EGF) Treatment on Multipotential Stromal Cells (MSCs). Possible Enhancement of Therapeutic Potential of MSC
Figure 1
Effect of EGF on primary human MSC proliferation (a) and accumulative population doubling (PD)(b). (a) The cell number of primary human MSCs increases about 5.5-fold in the diluent (Ctrl) in culture medium supplemented with 17% FBS in 120 hours (5 days) time period. The addition of EGF (10 nM) gives an extra increase of cell counts to 8.5-fold. Total of 10000 cells was seeded per each well in 12-well plate and the cell count of each well was measured by Coulter Cell Counter Z2 (Beckman Coulter, Inc. Fullerton, CA). Shown are mean s.e.m. of three experiments; each performed in triplicate. The differences in proliferation were compared between growth factor and diluent (Ctrl) exposed (*). (b) Accumulated PD of primary human MSCs in day 5 and 19 in the culture condition same as (a). After cell counting at day 5, equal number of cells (Total of 1000) was seeded per each well in 6-well plate and the cell count of each well was measured at day 19 by Coulter Cell Counter Z2 (Beckman Coulter, Inc. Fullerton, CA). Note that the PD is higher in EGF treated group (2.54 in Ctrl, 3.07 in EGF) for the initial 5 days (*), but this difference is reduced in the following 14-day period (10.79 in Ctrl, 11.19 in EGF)(No significant difference). Shown are representative data of three independent experiments; each performed in triplicate.
