Epidermal Growth Factor (EGF) Treatment on Multipotential Stromal Cells (MSCs). Possible Enhancement of Therapeutic Potential of MSC

<table>Effects of EGF treatment (10 nM) on primary human MSC colony formation. Five hundred cells were seeded in 10 cm dish within culture medium supplemented with 17% FBS and the number of formed colonies (Diameter <svg height="11.25" id="M13" style="vertical-align:-1.29163pt" version="1.1" viewbox="0 0 15.175 11.25" width="15.175" xmlns="http://www.w3.org/2000/svg">
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<tspan style="font-size: 12.50px; " x="0" y="0">≥</tspan>
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</svg>1.5 mm) was counted manually in day 14. (a) Representative image of MSC colonies stained with crystal violet. (b) Colony count of MSC. The number of colony is given per 1000 cells seeded initially (*<svg height="11.25" id="M14" style="vertical-align:-0.30096pt" version="1.1" viewbox="0 0 48.337502 11.25" width="48.337502" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.25)">
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<text transform="matrix(1,0,0,-1,-71.95,63.35)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝑃</tspan>
<tspan style="font-size: 12.50px; " x="10.890113" y="0">&lt;</tspan>
<tspan style="font-size: 12.50px; " x="22.930502" y="0">.</tspan>
<tspan style="font-size: 12.50px; " x="26.056252" y="0">0</tspan>
<tspan style="font-size: 12.50px; " x="32.307751" y="0">5</tspan>
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</svg> to EGF treatment). Shown are representative data of two independent experiments; each performed in triplicate.</table>

BioMed Research International

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Figure 2

Figure 2: Epidermal Growth Factor (EGF) Treatment on Multipotential Stromal Cells (MSCs). Possible Enhancement of Therapeutic Potential of MSC 