[Retracted] Gelsolin Restores Aβ-Induced Alterations in Choroid Plexus Epithelium

Figure 2

Secreted gelsolin inhibits A𝛽-induced disruption on choroid plexus epithelial cell cytoskeleton. (a) Representative confocal images of choroid plexus confluent monolayer labeled with anti-ZO-1 antibody. Under control conditions, ZO-1 immunostaining is distributed along the plasma membrane. In contrast, after exposure to A𝛽_1-42 for 48 hours, a disruption of the plasma membrane pattern of ZO-1 was observed, resulting in increased cytoplasmic localization. Note the ability of gelsolin treatment to prevent this A𝛽-induced alteration in ZO-1 pattern. (𝑛=3). (b) A𝛽_1-42 treatment resulted in increased serine phosphorylation of ZO-1 and decreased ZO-1 expression in choroid plexus epithelial cells. Gelsolin coadministration markedly attenuated A𝛽_1-42 alteration in ZO-1 (𝑛=3). (c) BIODIPY FL phallacidin staining of choroid plexus epithelial cells showed a disruption of the actin cytoskeleton after treatment with A𝛽_1-42 for 48 hours, and reversion when gelsolin was simultaneously added. Magnification: ×40. Scale bars = 10 𝜇m.

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