805405.fig.003a
(a)
805405.fig.003b
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805405.fig.003c
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805405.fig.003d
(d)
Figure 3: Secreted gelsolin expression modulates NO production and cell death in choroid plexus epithelial cells. (a) Choroid plexus epithelial cells treated with A 𝛽 1 - 4 2 for 48 hours exhibited a significantly enhanced NO production compared with untreated cells. Secreted gelsolin coadministration completely blocked this effect ( 𝑛 = 3 ); ** 𝑃 < . 0 1 . (b) Increased cell death was observed in choroid plexus cell cultures 48 hours after A 𝛽 1 - 4 2 treatment, and gelsolin addition totally reversed this toxic effect ( 𝑛 = 3 ); * 𝑃 < . 0 5 . (c and d) A 𝛽 1 - 4 2 treatment reduced mitochondrial complex IV in-gel activity in choroid plexus epithelial cells, whereas secreted gelsolin administration increased complex IV activity and reversed this decrease in A 𝛽 1 - 4 2 -induced activity. Blue native analysis of these culture samples showed altered protein expression in the mitochondrial complex IV. Representative blue native blots and quantitative histograms are shown ( 𝑛 = 4 per group); * 𝑃 < . 0 5 .