Identification of an MSI-H Tumor-Specific Cytotoxic T Cell Epitope Generated by the (−1) Frame of U79260(FTO)

Figure 4

Antibody-blocking ELISpot analysis. FSP11 recognition of FSP11-specific T cells could be blocked by antibodies towards HLA(pan), HLA-A0201, CD3, and CD8 but not by IgG2 control antibody. The reactivity without the addition of antibodies is given as positive control. T2 cells loaded with FSP11 were used as targets. Either T2 cells or T cells were preincubated with antibodies towards HLA(pan) and HLA-A0201 (T2) or CD3 and CD8 (T cells) for one hour prior to setup of the ELISpot.