The Bright and the Dark Sides of DNA Repair in Stem Cells

Frosina, Guido

doi:https://doi.org/10.1155/2010/845396

BioMed Research International

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The Complexities of Engineering Human StemCell-Derived Therapeutics

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Review Article | Open Access

Volume 2010 | Article ID 845396 | https://doi.org/10.1155/2010/845396

The Bright and the Dark Sides of DNA Repair in Stem Cells

Guido Frosina¹

Academic Editor: James A. Ross

Received28 Aug 2009

Revised16 Nov 2009

Accepted01 Feb 2010

Published08 Apr 2010

Abstract

DNA repair is a double-edged sword in stem cells. It protects normal stem cells in both embryonic and adult tissues from genetic damage, thus allowing perpetuation of intact genomes into new tissues. Fast and efficient DNA repair mechanisms have evolved in normal stem and progenitor cells. Upon differentiation, a certain degree of somatic mutations becomes more acceptable and, consequently, DNA repair dims. DNA repair turns into a problem when stem cells transform and become cancerous. Transformed stem cells drive growth of a number of tumours (e.g., high grade gliomas) and being particularly resistant to chemo- and radiotherapeutic agents often cause relapses. The contribution of DNA repair to resistance of these tumour-driving cells is the subject of intense research, in order to find novel agents that may sensitize them to chemotherapy and radiotherapy.

1. Introduction

Endogenous damage (e.g., oxidative metabolism linked) and external exposures (e.g., environmental pollution linked) all damage DNA causing a number of modifications including base and backbone alterations, single strand breaks (SSB) and double strand breaks (DSB) that may limit survival and the regenerative potential of both embryonic stem cells (ESC) and adult stem cells (ASC). ESC differentiate to all cell types in the mammalian body, including germ line cells. The maintenance of genomic stability in ESC must be stringent, any genetic alterations in those progenitor cells compromising the genomic stability and functionality of entire cell lineages. Consistently, the mutation rate and the frequency of mitotic recombination are lower in murine ESC than in adult somatic cells or isogenic mouse embryonic fibroblasts (MEF). For instance, the frequency of spontaneous mutation at the aprt gene is around 10 in ESC and 100-fold higher (~10) in MEF [1]. Mechanisms of mutagenesis differ as well. Most mutation events involve loss of heterozigosity (LOH) in both ESC and MEF but LOH is generated mainly through nondisjunction in ESC and through mitotic recombination in MEF [2]. Similarly, when spontaneous mutation is assessed at the X-linked locus hprt, it is undetectable in ESC (<10) and ~10 in MEF. Hence, robust mechanisms counteracting spontaneous mutagenesis may exist in ESC and DNA repair is likely one of them [2–6]. On the other hand, ASC are important in the long-term maintenance of tissues throughout life [7]. For instance, the effector cells of the blood have limited lifetimes and must be replenished continuously throughout life from a small reserve of hematopoietic stem cells in the bone marrow. Although the replicative potential of hematopoietic stem cells may be finite, studies conducted in murine genetic models indicate that DNA repair is critical to the longevity and stress response of the hematopoietic stem cell pool [8]. This likely applies to other ASC types including mesenchymal stem cells (MSC) [9].

2. The Bright Side: DNA Repair in Normal Stem Cells

2.1. ESC

Using single-cell gel electrophoresis (SCGE) Maynard and coworkers [5] found that human ESC have more efficient repair of different types of DNA damage [generated from HO, ultraviolet (UV)-C, ionizing radiation (IR) or psoralen] than human primary fibroblasts and, with the exception of UV-C damage, HeLa cells (Table 1). A microarray gene expression analysis showed that the mRNA levels of several DNA repair genes, including some involved in DNA base excision repair (BER) and interstrand crosslink (ICL) repair, were elevated in human ESC compared with their differentiated forms [embryoid bodies (EB)]. Hence, multiple DNA repair pathways are over-regulated in human ESC, relative to differentiated human cells [5]. Consistently, the expression of antioxidant and DNA repair genes was reduced and the DNA damage levels increased during spontaneous differentiation of two human ESC lines [10]. Replicating chromatin in ESC is particularly vulnerable to strand breaks [11]. Two pathways seal DSBs in mammalian cells, including ESC: non homologous end joining (NHEJ) that is the main pathway and homology-directed repair (HDR) that may act as a backup in the absence of NHEJ components [12]. The expression of strand break repair genes such as Rad51 fades when murine ESC differentiate [2, 10, 13] (Table 1). Despite their high expression of O methylGua DNA methyltransferase (MGMT), murine ESC undergo apoptosis at much higher frequency than differentiated cells after treatment with N-methyl-N-nitro-N-nitrosoguanidine (MNNG) [14] (Table 1). This is due to elevated expression in ESC of the mismatch repair (MMR) proteins MSH2 and MSH6 that trigger futile cycles of O methylguanine repair/replication [15]. It has been hypothesized that the high apoptotic response of murine ESC may contribute to reduce the mutational load in these cells [14, 16, 17] (Table 1). Embryonic Fanconi anemia (FA) neural stem cells (NSC) have a reduced capacity to self-renew in vitro [18, 19] and the expression of FA G and FA L (but not FA C) genes is enhanced in human ESC relative to human EB [5]. In summary, 7 out of 9 (78%) studies indicate that ESC possess more elevated DNA repair capacity than their differentiated derivatives (Table 1).

2.2. ASC

The repair capacity for DNA strand breaks diminishes during maturation of cells of the human lymphohematopoietic system. Bracker and coworkers [20] have studied the variation of DNA repair capacity and expression of DNA repair genes during maturation of hematopoietic cells treated with ENU or melphalan (Table 1). The removal of DNA adducts, the resealing of strand breaks and the resistance to DNA-reactive drugs were higher in stem (CD34+ 38−) than in mature (CD34−) or progenitor (CD34+ 38+) cells isolated from umbilical cord blood from the same individual [20]. Hence, slow dividing stem cells may be protected by extensive DNA repair while more mature and less valuable cells, if damaged, could be rather eliminated by apoptosis. The NHEJ components such as the Ku70 protein are downregulated during ageing of the hematopoietic stem cell donor. Ku70 expression shows highest levels in newborn, 2.6-fold lower levels in young (mean age: 30 years) and 6.3-fold lower levels in old (mean age: 87.6 years) donors [21]. Transcription-coupled repair (TCR) is a subpathway of nucleotide excision repair (NER) that preferentially repairs the transcribed strand of active genes, as compared to the non-transcribed strand, thus providing cells with a smart mechanism of safeguard of expressed genes [37]. Likewise strand break repair and BER, NER is strongly downregulated in several human cell types undergoing differentiation (e.g., in cells of the monocytic lineage when they differentiate to macrophages or NSC differentiating to neurons) [23, 24, 38] (Table 1). Attenuation of NER during differentiation results from lack of ubiquitination of NER proteins that in turn is linked to differences in phosphorylation of the ubiquitin-activating enzyme E1 [24]. To ensure proficient repair of active genes, besides TCR, a second specialized mechanism termed differentiation-associated repair (DAR) exists in differentiated cells. DAR recruits to transcribed genes of differentiated cells the remaining proficient NER enzymes not yet engaged in TCR that eventually repair both DNA strands [24]. Hence, DAR may be considered as a subpathway of global genome NER, focussing on the chromatin domains of differentiated cells within which transcription occurs [22, 24]. In human hematopoietic cord blood cells, Casorelli et al. [25] found no difference in sensitivity to methylating agents between cycling CD34+ (stem) and CD34− (mature) cells (Table 1). In this study, MGMT significantly protected against N-Methyl-N-Nitrosourea (MNU) toxicity while MMR enhanced as expected the MNU sensitivity of the cells by processing O-methylguanine into a lethal lesion [15, 25]. The important role of DSB repair in maintenance of hematopoietic stem cell function has been recently emphasized in two murine studies [8, 39, 40]. Progressive loss of hematopoietic stem cells and a decrease of bone marrow cell count were observed in mice with defective DSB repair and stem cell function in tissue culture and transplantation was severely impaired [40]. Stem cells in the central nervous system (CNS) might behave differently. No resistance of neural precursors to IR was observed in two studies [26, 27] (Table 1). Nowak and coworkers [26] reported massive apoptosis of neural precursors but not of neurons in the developing mouse brain after gamma-irradiation while the level of induced damage was similar in the two cell types. In the second study, Panagiotakos et al. [27] have observed a specific association between radiation injury and irreversible damage to stem cells in the subventricular zone (SVZ) and loss of oligodendrocyte progenitor cells (OPC) in both rodent and human brain. Hence unlike hematopoietic precursors, DSB repair might be less efficient in the neural precursors in relation to their greater radiosensitivity. Expression of 8-oxoguanine DNA glycosylase (OGG1) is elevated in regions of the neonatal mouse brain that are rich in neural stem/progenitor cells, namely the medial wall of the lateral ventricle and the SVZ [28] (Table 1). Both the expression and the activity of OGG1 are high in neural stem/progenitor cells from newborn mice and decrease in adult animals and upon induction of cell differentiation. Enhanced expression of OGG1 and other BER enzymes may protect neural stem/progenitor cells from oxidatively damaged DNA [28]. Murine Nei endonuclease VIII-like 3 (Neil3) glycosylase follows as well an expression pattern involving brain regions harbouring stem cell populations [29, 30] (Table 1). The glycosylase activities are stable through prolonged in vitro culture required for expansion of stem cells to clinically relevant numbers [41]. Downregulation of oxidatively damaged DNA repair genes and a concomitant increase in 8-oxoguanine (8-oxoGua) DNA levels during differentiation of mouse proliferating (myoblasts) to terminally differentiated (myotubes) muscle cells have been described by Narciso and coworkers [32] (Table 1). Both short and long patch BER pathways were impaired in myotubes. The defect in BER was linked to the nearly complete lack of DNA ligase I and to the strong down-regulation of XRCC1 with consequent destabilization of DNA ligase III [32]. The FA pathway exerts a central role in neural stem and progenitor cells during developmental and adult neurogenesis [18, 19]. Reduced proliferation of neural progenitor cells and enhanced NSC depletion has been observed in ageing FA mice. Lower reactive oxygen species (ROS) levels may be achieved in NSC by higher expression of key antioxidant enzymes involved in basal mitochondrial metabolism (e.g., uncoupling protein 2 (UCP2) and glutathione peroxidase (GPX)) as compared to postmitotic neural cells [42]. Following exposure to the mitochondrial toxin 3-nitropropionic acid and unlike postmitotic cells, NSC fastly upregulate UCP2, GPX and superoxide dismutase 2 and successfully recover. Thus, a fast response of antioxidant enzymes may represent an important “vigilance” mechanism of NSC to counteract oxidatively damaged DNA in the CNS. Summary reckoning indicates that in 8 out of 13 (61%) studies, ASC display more elevated DNA repair capacity than mature cells (Table 1; [31, 33]).

2.3. MSC

The gene expression profiles of undifferentiated MSC derived from first trimester fetal liver and adult bone marrow were compared by serial analysis of gene expression, and validated by either reverse transcription polymerase chain reaction or immunoblotting of selected genes [34]. Transcripts implicated in cell cycle promotion, chromatin regulation and DNA repair were more abundant in fetal than in adult MSC (Table 1). Likewise, MSC obtained from bone marrow transplantation patients display increased DSB repair capacity and resistance to IR and possess elevated ROS-scavenging capacity as compared to lung cancer and breast cancer cells [35] (Table 1). Telomerase activity is an additional mechanism by which MSC may resist IR damage. Telomerase-immortalized derivatives of human MSC have been found IR-resistant as compared to primary stem cells while DNA repair capacity was similar in the two cell types [36] (Table 1). Considering the aforementioned study by Galderisi and coworkers on senescence of MSC [9], 4 out of 5 (80%) studies indicate that DNA repair is elevated in MSC although appropriate comparison to differentiated cells is not available.

3. The Dark Side: DNA Repair in Cancer Stem Cells

Stem cells and cancer cells share some features: similar signalling pathways may regulate self-renewal in stem cells and cancer cells, and cancer cells may include “cancer stem cells”—rare cells with indefinite potential for self renewal and differentiation that drive tumorigenesis [56]. A detailed knowledge of the biological distinctness of cancer stem cells may be crucial for the development of specific therapies aimed to tumour eradication [57–59]. In particular, the existence of cells endowed with features of primitive progenitor cells and tumor-initiating function has been demonstrated in high grade gliomas [60] (Figure 1).

Figure 1

Resistance in GSC. Normal NSC self-renew and give rise to multipotential progenitor cells that form neurons, oligodendroglia, and astrocytes. GSC arise from the transformation of either NSC or progenitor cells (red) or, less likely, from de-differentiation of oligodendrocytes or astrocytes (thin red arrows) and lead to malignant gliomas. GSC are relatively resistant to standard treatments such as radiation and chemotherapy and lead to regrowth of the tumor after treatment. Therapies directed at stem cells can deplete these cells and potentially lead to more durable tumor regression (blue) (from [60] with permission).

3.1. High Grade Gliomas

Despite aggressive surgical resections using preoperative and intraoperative neuroimaging, along with recent advances in radiotherapy and chemotherapy, the prognosis for high grade glioma patients remains dismal, the median survival being 24–60 months for patients with anaplastic astrocytoma (World Health Organization (WHO) grade III) and 12–15 months for patients with glioblastoma multiforme (WHO grade IV) [60, 61]. Established prognostic factors are limited and include age at diagnosis, Karnofsky performance status, extent of surgery and possibly MGMT promoter methylation [60, 62]. Standard treatment includes resection of >95% of the tumor, followed by concurrent chemotherapy (usually performed with the alkylating agent temozolomide (TMZ)) and radiotherapy. Malignant gliomas are associated with such dismal prognoses in part because glioma cells can actively migrate through the brain, often travelling relatively long distances, making them elusive targets for effective surgical removal and almost invariable sources of relapse [63]. In children, the management of high grade gliomas remains an even greater challenge for neuro-oncologists in part because of the greater vulnerability of the developing brain to treatment-related toxicity [64].

3.2. Origins of Glioma Stem Cells

Although the brain is completely formed and structured few weeks after birth, it maintains a degree of plasticity throughout life, including axonal remodeling, synaptogenesis, but also neural cell birth, migration and integration [65]. The SVZ and the dentate gyrus of the hippocampus are the two main neurogenic sites in the adult brain. NSC reside in these structures and produce progenitors that possess migratory ability. One current fashionable model suggests that gliomas may arise from the transformation of neural stem or progenitor cells, originating cancer cells that are undifferentiated, self-renewing, with the capacity for driving tumor development and designated glioma stem cells (GSC), because of their stem-like properties. The origin of GSC has been investigated by activating oncogenic K-RAS in mouse neuronal precursor cells and adult SVZ cells [66]. K-RAS-activated mice showed a marked expansion of stem cell populations in the SVZ and developed intermediate grade, infiltrating glioma with 100% penetrance. Tumors were consistently located in the amygdalohippocampal region and nearby cortex and tumor cells expressed markers associated with neural progenitor cells, including OLIG2, BMI1, and platelet-derived growth factor (PDGF) receptor-alpha. Therefore, infiltrating tumor cells arose in this study from NSC transformed by oncogenic activation in vivo [66]. In a different approach, a mouse strain where tumor induction would be restricted to myelinating OPC was generated [67]. PDGF-B transfer to OPC could induce gliomas with an incidence of 33%. The majority of tumors resembled human WHO grade II oligodendroglioma based on close similarities in histopathology and expression of cellular markers. Thus, in this system OPC could act as cell of origin for experimental glioma [67]. The generation of mouse gliomas following the overexpression of PDGF-B in embryonic neural progenitors has been described by Appolloni et al. as well [68]. Histopathological, immunohistochemical and genome-wide expression features of PDGF-B induced tumors were surprisingly uniform, despite they were generated by transducing a highly heterogeneous population of progenitor cells known for their ability to produce all the cell types of CNS. This uniformity is likely due to the ability of PDGF-B overexpression to respecify competent embryonic NSC toward the oligodendroglial lineage. PDGF-B-induced tumors harbored different proliferating cell populations but only PDGF-B-overexpressing cells were tumorigenic [68]. The possibility that GSC originate in some cases from de-differentiation of tumoural cells cannot yet be ruled out [69, 70]. It has been recently observed that the reversion of mature astrocytes to an embryonic state is sufficient to sensitize them to oncogenic stress [71]. Prolonged exposure of astrocytes to transforming growth factor (TGF)-alpha is sufficient to trigger their reversion to a neural progenitor-like state (de-differentiated astrocytes). When dedifferentiated astrocytes were grafted intra-cerebrally, they showed the same cytogenomic profile as astrocytes, survived in vivo and did not give birth to tumors. After exposure to IR yet, they acquired cancerous properties: they were immortalized, showed cytogenomic abnormalities, and formed high-grade glioma-like tumors after brain grafting [71]. Anyway, whatever the GSC origin, malignancy of gliomas often correlates with the stem phenotype [43–47, 50, 51] (Table 2) and accordingly, if only a subset of glioma cells drives tumour progression it is important to target it specifically (Figure 1) [60, 72, 73].

3.3. What are GSC?

Although methods of deriving GSC from glioma tumours and sorting them according to certain marker expression (CD133, Nestin, Sox-2 and Musashi-1) have been described [50, 75, 76], the exact identity of GSC remain elusive [70]. The most extensive of the limited data on this topic relate to GSC identified by the surface marker CD133 (Table 2). The stem cell marker CD133/prominin-1 is a a five transmembrane domain glycoprotein which has been identified as a cancer stem cell marker in several solid tumor types, including those of the brain. CD133 is often expressed on the surface of human GSC and CD133-positive cells may represent >85% of certain human and animal glioma cell lines [55, 77]. To verify if CD133-expressing cells are essential for tumorigenesis, a glioma-initiating mouse cell line whose CD133-positive cells can be eliminated conditionally by a Cre-inducible diphtheria toxin fragment A (DTA) gene on the CD133 locus, was generated by Nishide and coworkers [53]. After induction of the DTA gene, the cell line maintained the capacity to form tumour spheres culture and transplantable multiform glioblastoma (GBM) in vivo. Hence at least in this mouse model, CD133-expressing cells are dispensable for gliomagenesis [53]. The presence of two types of GSC within different regions of the same human GBM supports this conclusion [48]. Cytogenetic and molecular analysis showed that the two types of GSC bore quite diverse tumorigenic potential and distinct genetic anomalies, and yet, CD133 expression was similar. Those two GSC populations might represent distinct cell targets, with a differential therapeutic importance independently of CD133 expression [48]. CD133 failed to identify the total cancer stem-like cell population in the rat C6 glioma cell line, since both CD133-positive and CD133-negative cells displayed cancer stem-like cell fractions showing characteristics of self-renewal, multilineage differentiation potentials in vitro, and tumorigenic capacity in vivo [54]. Methodological problems concerning purification of CD133-expressing cells may exist. It has been recently reported that the specific expression and enrichment of CD133 can be obtained in fresh human gliomas and gliomasphere cultures purified by fluorescence activating cell sorting while purification of CD133-positive GSC using the widely used CD133-microbeads may be affected by lack of specificity and lead to mixed populations [78]. In conclusion, CD133-positive GSC likely drive only an as yet unquantified subset of malignant gliomas, the remainder deriving from CD133-negative GSC with distinct phenotypical features [46, 79]. The neurosphere formation ability and tumorigenic capacity of cultured glioma cells have been recently explored as independent predictors of patient’s outcome [52]. Those features of cultured glioma cells significantly correlated with an increased hazard of patient’s death and more rapid tumor progression independently of Ki67 proliferation index [52]. These results suggest that the ability to propagate brain tumor stem cells in vitro is associated with clinical outcome although the lengthy duration of this assay may preclude direct clinical application. The ultrastructural features of GSC isolated from both a primary glioma tissue and the human glioma cell line SHG-44 have been described [80]. The ultrastructural features of the two kinds of GSC were similar, with relatively developed mitochondria, Golgi apparatuses, ribosomes, undeveloped rough endoplasmic reticula, rare lysosomes and no typical autophagosomes, and high nuclear-cytoplasmic ratio. Their nuclei, frequently containing huge amounts of euchromatin and a small quantity of heterochromatin, were mostly globular and the majority of them had only one nucleole. Typical apoptotic cells could hardly be found in tumor spheres, and between adjacent cells there were cell junctions, which probably were incompletely developed desmosomes or intermediate junctions [80]. A definite (and sometimes disregarded) feature of GSC is their ability to differentiate. This is the only parameter that unequivocally permitted, in our experience, to distinguish stem from nonstem glioma cells [77]. In this study, stem cells cultured as adherent monolayers conveniently allowed morphologic analysis of differentiation under the inverted light microscope. Removal of growth factors and addition of foetal calf serum (FCS-differentiating conditions) resulted after 3 weeks in acquisition of a typical astroglial morphology by genuine GSC. Recent evidence shows that even single GSC possess multilineage potential [81]. Analysis of marker expression usually confirms the morphologic analysis. For instance glial fibrillary acidic protein (GFAP) is an intermediate filament protein that is typical, although not exclusive, of cells of astroglial lineage and may indicate that differentiation of stem cultures is mostly oriented to astroglial commitment. In a sense, nothing is better than visual inspection for morphological changes upon growth factors removal to determine whether cells are bona fide stem or not. Tumourigenic glioma cells unable to acquire any of the astrocyte, neuron, or oligodendrocyte morphology upon growth factors removal may be called “tumour initiating cells” or “tumour-driving cells” but not tumour stem cells, even if they express so-called “stemness” markers.

3.4. Deregulated Pathways in GSC

Invasive malignant glioma cells often show a decrease in their proliferation rates and a relative resistance to apoptosis that may underlie their resistance to conventional chemotherapy and radiotherapy [61]. Invasive growth and resistance to apoptosis results from changes at the genomic, transcriptional and post-transcriptional level of a number of cellular factors involved in complex signal pathways (reviewed in [74]) (Figure 2). For instance, the proliferation of normal stem and progenitor cells in the brain is under control of p53 [84]. The altered expression of several cell cycle regulators, in particular a pronounced downregulation of p21, has been observed in p53-mutant NSC indicating that p53 may act as a growth suppressor of GSC [85]. Deregulation of a number of additional cell cycle control pathways, including the p16-CDK4-RB pathway may underlie the generation of GSC in the brain [86, 87]. Activation of signaling pathways like the PDGF pathway [88], often accompanied by Ras inactivation [89], has been implicated in transformation of SVZ NSC (Figure 2). Another contributor is the Sonic Hedgehog (Shh) pathway that regulates the patterning, proliferation and survival of NSC within the CNS [90]. Shh signalling is mediated by Gli1 [91] that likely serves as a protective mechanism against premature mitosis in normal NSC. Deregulation of Gli1 has been observed in GSC [91] (Figure 2). Similarly, the WNT Notch and TGF-beta/Bone Morphogenetic Protein (BMP) developmental pathways have been also found aberrantly expressed in GSC [92]. Secretion of the angiogenic factor vascular endothelial growth factor (VEGF) by GSC has been observed and this phenomenon is further induced by hypoxia [93, 94] (Figure 2). Monoclonal antibodies and low molecular-weight kinase inhibitors of some of the above pathways may be of help in targeting GBM. For instance, the anti-VEGF neutralizing antibody bevacizumab limits the proangiogenic effects of GSC and may suppress the growth of GSC-derived xenografts in some cases [95]. However, most clinical trials of these agents as monotherapies have failed to demonstrate significant survival benefit, likely linked to the complexity of GBM biology that is characterized by a constantly changing microenvironment that greatly influences both tumor growth and response to therapy [63, 96].

Figure 2

Complex signal pathways and cellular factors regulate GSC. GSC are controlled at multiple levels by complicated regulatory networks. Signals initiated by receptor tyrosine kinases (RTK), bone morphogenetic protein receptors (BMPR), Hedgehog, and Notch result in complicated intracellular events to help balance self-renewal and differentiation of GSC as well as the promotion of cell survival and proliferation. Intracellular regulators including transcriptional factors (Olig2, Myc, Oct4, etc.), epigenetic modifiers (Bmi1), and microRNAs are also highly potent of maintaining GSC populations due to their ability to regulate massive downstream targets simultaneously (from [74] with permission).

3.5. DNA Repair as a Resistance Mechanism in GSC

Whether DNA repair is a major mechanism of resistance to apoptosis in cancer stem cells, likewise normal stem cells, still is an open question [6, 97]. For instance, myeloid progenitor bone marrow cells derived from BER-defective mice display unexpected bone marrow alkylation resistance as compared to progenitor cells from wild-type mice [98]. Hence, in this case, repairing the damaged base seems more lethal than leaving it unprocessed. The phosphatidylinositol-3-kinase (PI3K)-Akt pathway, a protumorigenic signaling cascade involved in several human cancers, is frequently up regulated in gliomas (Figure 2) (reviewed in [99]). Hyperactivation of the PI3K-Akt pathway occurs in gliomas through a variety of mechanisms, including loss of the inhibitory effects of the phosphatase and tensin homolog (PTEN) tumor suppressor [100]. Enhanced Akt signaling may cause a tumourigenic phenotype with increased cell proliferation, metastasis and angiogenesis. Akt inhibitors may significantly reduce viability of GSC relative to matched non stem cells [101] and sensitize them to chemotherapeutic agents [102]. Inhibiton of the Akt pathway further causes delayed repair of IR-induced DSB detected by gamma-H2AX foci formation and radiosensitization [103]. Hence activation of the Akt signaling may underlie at least some cases of radioresistance in GSC. Another route involves the activation of the DNA damage checkpoint response. Tumour cells expressing CD133 increase in gliomas treated with IR. CD133-positive GSC are more resistant than CD133-negative cells to IR in both in vitro experiments or when expanded in the brains of NOD/SCID mice and activate the DNA damage checkpoint response with unusual intensity [104]. Increased DNA repair capacity, evaluated by the SCGE assay has been also reported [104, 105]. Debromohymenialdisine (DBH), a specific inhibitor of the Chk1 and Chk2 checkpoint kinases, delayed the checkpoint response and reversed the resistant phenotype [104]. Similarly, IR-mediated phosphorylation of Chk1 and Chk2 have been found distinctly higher in undifferentiated human embryonal carcinoma cells compared with differentiated cells [106]. G2 and G1-arrested NSC rapidly increase after IR in the mouse, with concomitant phosphorylation of cdc2 and p53 and inhibition of Notch [107]. Hence, although the selective resistance of GSC has been questioned [108], CD133-positive cells may represent the cellular population that resists to IR and chemotherapy at least in a subset of glioma tumours and activation of the DNA damage checkpoint response may be a major underlying mechanism in this regard [49, 77, 104]. On the contrary, we could not confirm enhanced DNA repair capacity in GSC [77]. It should be pointed out that the concept “enhanced DNA repair capacity” implies “per time unit”. Five GSC lines isolated from primary grade IV gliomas were examined for their cell cycle and DNA repair features [77]. The population doubling time of the GSC lines was significantly longer as compared to nonstem cell lines while no enhanced DNA repair examined by an in vitro BER assay, the SCGE assay or resolution of pH2AX nuclear foci, could be observed. The DNA damage checkpoint response was constitutively activated in CD133-positive but not in CD133-negative cells, with Chk1 and Chk2 kinases phosphorylated in the absence of treatment. Recent studies indicate cell cycle restriction as a mechanism of resistance in leukaemia as well [109]. Hence, constitutive activation of the DNA damage checkpoint response may confer GSC increased time for the removal of radio- and chemotherapeutic- induced DNA lesions before arrival of the replication fork while whether improved efficacy of repair (per time unit) is a common resistance mechanism in GSC still requires further studies.

3.6. Targeting the Checkpoint Response in GSC

Once DNA damage is identified with the aid of sensors, the checkpoint transducers ATM (ataxia-telangiectasia mutated) and ATR (ATM and Rad3-related) proteins undergo conformational change and/or localisation, resulting in their activation. ATM and ATR phosphorylate the downstream effectors checkpoint kinase-2 (Chk2) and checkpoint kinase-1 (Chk1) respectively, two serine/threonine kinases that serve as functional analogues (reviewed in [110, 111]) (Figure 3(a)). Significant crosstalk exists between the two pathways [112]. Sites of Chk2 phosphorylation by ATM include Ser 33/35, Thr68, Ser19, Thr387 and Thr432 while ATR phosphorylates Chk1 mainly at serines 280, 296, 317 and 345. The first and second-level transducers ATM, ATR, Chk1 and Chk2 phosphorylate a number of effector molecules, such as p53 and CDC25 phosphatases. In particular, CDC25 phosphatases are key checkpoint kinase targets for controlling cell cycle transitions. Human cells have three CDC25 proteins that regulate cell cycle transitions by removing the inhibitory phosphorylation from cyclin-dependent kinases (CDKs). Chk1 and Chk2 phosphorylation of the CDC25 proteins inhibits their activity through either ubiquitin-mediated degradation or cytoplasmic sequestration and prevents CDK activation. This negative regulation of the CDC25 phosphatases is a major checkpoint mechanism for entry of cells into mitosis.

(a)

(b)

Figure 3

Cell cycle checkpoint pathways, possible targets in GSC. (a) Once DNA damage is identified with the aid of sensors, the checkpoint transducers ATM and ATR undergo conformational change and/or localisation, resulting in their activation. ATM and ATR activate a series of downstream molecules, including the checkpoint kinases Chk1 and Chk2. The latter inactivate CDC25 phosphatases, culminating in cell cycle arrest. AZD7762 (AstraZeneca) and DBH are specific inhibitors of Chk1 and Chk2 kinases. CP466722 (Pfizer) is a specific inhibitor of ATM (modified from [82] with permission). (b) Targeting GSC may yield durable tumor regression. Glioblastomas are heterogeneous tumours containing CD133-positive GSC among other, more differentiated, CD133-negative cells, including glioblastoma progenitor cells. Following radiation, the bulk glioblastoma responds and the tumour shrinks but CD133-positive cells activate checkpoint controls for DNA repair more strongly than CD133-negative cells, resist radiation and prompt the tumour to regrow. These cells could be targeted with DNA-checkpoint blockers (e.g., AZD7762, CP466722 and DBH) to render them radiosensitive (modified from [83] with permission).

Releasing the constitutively-pressed brake that prevents GSC from dividing may push them into cell cycle and sensitize them to IR and chemotherapeutic agents such as TMZ that primarily act by damaging DNA [82]. To this purpose, specific inhibitors for the key actors of the checkpoint response namely ATM, ATR, Chk1 and Chk2 are the object of intense academical and industrial research [113]. One possible candidate could be AZD7762 developed by Zabludoff and coworkers at AstraZeneca [114]. AZD7762 is a potent ATP-competitive checkpoint kinase inhibitor that was shown to potentiate the cytotoxicity of DNA-damaging drugs towards different types of tumours cultivated in vitro, by abrogating the DNA damage checkpoint response. Importantly, the potentiation was observed in vivo as well, using multiple xenograft models and several DNA-damaging agents, indicating that the drug could be worth exploring in the clinical setting to increase patient’s response rates. Another potentially interesting drug is CP466722 developed by Rainey and coworkers [115]. These authors identified CP466722 as a potent and specific ATM inhibitor after screening a targeted compound library. Inhibition by CP466722 abrogated the ATM-dependent phosphorylation activity and the cell cycle checkpoint response and could be reversed by removing the drug. HeLa and AT GM02052 cells were sensitized to IR in the presence of CP466722 in vitro. No in vivo experiments were reported in this study. A number of additional cell cycle checkpoint inhibitors are available (e.g., the aforementioned DBH) or under development [113]. Their use could permit significant sensitization of GSC to radiotherapy and chemotherapy.

4. Conclusions

Enhanced DNA repair capacity is often observed in normal (embryonic or adult; human or murine) stem cells as compared to differentiated cells, suggesting that normal stem cells often protect their genome through enhanced DNA repair. This may not be the case for cancer stem cells. At least in gliomas, DNA repair rates are normal but low proliferation and constitutive activation of the DNA damage checkpoint response confer increased time for lesion removal or bypass before arrival of the replication fork. Hence, GSC do not repair DNA better. They just have more time to do that. Those features may be common to stem cells from other tumour types as well [109]. Drugs targeting cell cycle restriction in GSC could be of help for complete eradication of the tumor and several novel agents of this kind are under development. In particular ATM and Chk1 and Chk2 kinase inhibitors may effectively sensitize GSC to IR and alkylating agents by stimulating their proliferation.

Abbreviations

ASC:	Adult stem cell(s)
ATM:	Ataxia-telangiectasia mutated
ATR:	ATM and Rad3-related kinase
BER:	DNA base excision repair
BMP:	Bone morphogenetic protein
BMPR:	Bone morphogenetic protein receptor
CDK:	Cyclin-dependent kinase
Chk1:	Checkpoint kinase-1
Chk2:	Checkpoint kinase-2
CNS:	Central nervous system
DAR:	Differentiation-associated repair
DBH:	Debromohymenialdisine
DSB:	Double-strand break(s)
DTA:	Diphtheria toxin fragment A
EB:	Embryoid body
ESC:	Embryonic stem cell(s)
FA:	Fanconi anemia
FCS:	Foetal calf serum
GBM:	Multiform glioblastoma
GFAP:	Glial fibrillary acidic protein
GPX:	Glutathione peroxidise
GSC:	Glioma stem cell(s)
HDR:	Homology-directed repair
ICL:	Interstrand crosslink
IR:	Ionizing radiation
LOH:	Loss of heterozigosity
MEF:	Mouse embryonic fibroblast(s)
MGMT:	O methylGua DNA methyltransferase
MMR:	Mismatch repair
MNNG:	N-methyl-N-nitro-N-nitrosoguanidine
MNU:	N-methyl-N-nitrosourea
MSC:	Mesenchymal stem cell(s)
Neil3:	Nei endonuclease VIII-like 3 glycosylase
NER:	Nucleotide excision repair
NHEJ:	Non homologous end joining
NSC:	Neural stem cell(s)
OGG1:	8-oxoGua DNA glycosylase
8-oxoGua:	8-oxoguanine
OPC:	Oligodendrocyte progenitor cell(s)
pol:	DNA polymerase
PI3K:	Phosphatidylinositol-3-kinase
PDGF:	Platelet-derived growth factor
PTEN:	Phosphatase and tensin homologue
ROS:	Reactive oxygen species
RTK:	Receptor tyrosine kinase
SCGE:	Single cell gel electrophoresis
Shh:	Sonic Hedgehog
SSB:	Single strand break(s)
SVZ:	Subventricular zone
TCR:	Transcription-coupled repair
TGF:	Transforming growth factor
TMZ:	Temozolomide
UCP2:	Uncoupling protein 2
UV:	Ultraviolet
VEGF:	Vascular endothelial growth factor
WHO:	World Health Organization.

Acknowledgments

The work is partially supported by Compagnia di S. Paolo, Turin, Italy (Progetto “Sensibilizzare i tumori cerebrali alla radio- e chemioterapia con inibitori dei checkpoint del ciclo cellulare”) and Istituto Superiore Sanità, Rome, Italy (Programma Italia-USA “Malattie Rare”).

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Copyright © 2010 Guido Frosina. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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