Size of AT_(𝑛) Insertions in Promoter Region Modulates Gmhsp17.6-L mRNA Transcript Levels

Figure 5

Gmhsp176-L promoter region alignment from the resistant parental, PI595099, susceptible parental, BRS133, and fragments from susceptible offsprings 256-S, 259-S (heterozygous individual) and 266-S and from resistant offsprings JF7002, JF7027 and JF7056. SOYHSP176_11317-Gmhsp176-L gene sequence, available at the Genbank. PCR amplification was conducted with primers, which delimitated promoter region. In light gray, AT_(n) insertion, in dark gray some heat shock elements, with consensus core sequences 5^′AGAAnnTTCT3^′, 5^′cTTCtaGAAgcTTCtaGAAg3^′, and 5^′CTnGAAnnTTCnAG3^′ and underlined probable TATA box.