Receptor-Mediated and Fluid-Phase Transcytosis of Horseradish Peroxidase across Rat Hepatocytes
Figure 3
Effect of mannan on biliary HRP secretion. Livers were isolated and following 30 minutes perfusion, HRP (0,8 mg/mL) plus 1.6 mg/mL mannan was administered in the perfusion medium (KHB) for 1 minute at 37°C (time 0) followed by single-pass perfusion with marker-free KHB at 37°C for 40 minutes. The amount of HRP in single bile drops was determined and the secretion rate was calculated (a) based on the bile flow (b). Data shown are the mean SD of four perfusions. (c) The total amount of HRP (cummulative HRP secretion) secreted into bile as a function of the perfusion time is shown.
