Figure 1: Effect of pRBC on the release of ET-1 and big ET-1 from human microvascular endothelial cells (HMECs-1). HMECs-1 were treated for 24 hours in the presence of normal RBC or pRBC (D10 or W2 strains) at 2–4% parasitaemia in (a), (c), (d) or at the indicated levels of parisitaemia (b) under normoxic or hypoxic conditions. Supernatants were then assayed for the presence of ET-1 (a, b) or big ET-1 (c) by ELISA. Results represent the mean ± SD from five different experiments. (a) and D10 versus control in normoxia and hypoxia, respectively; and W2 versus control in normoxia and hypoxia, respectively; (b) and : D10 8% and 4% versus control, in normoxia; : D10 8%, 4%, and 2% versus control in hypoxia. (d) RT-PCR analysis of ET-1. G3 PDH served as control. The cells were lysed and mRNA extracted. The PCR products were separated through agarose gel electrophoresis and visualized by ethidium bromide. The reported data are representative of three independent experiments.