854927.fig.002a
(a)
854927.fig.002b
(b)
Figure 2: Effect of incubation of pRBC (D10 strain) or normal RBC on synthetic ET-1 and big Et-1. (a) Different concentrations of ET-1 (square) or big ET-1 (triangle) were incubated in medium (empty symbols) or in the presence of uninfected RBC (grey symbols) or pRBC (black symbols). After 24 hours of incubation, samples were collected and assayed for the presence of the residual ET-1 or big et-1 by ELISA. The results represent the mean ± SD from three different experiments. (b) ET-1 (5 fmol/mL) was incubated in medium (empty square) or in the presence of pRBC (black square) or control RBC (black triangle) for 2, 4, 6, 24 hours. At the end of incubation, samples were collected and assayed for the presence of the residual ET-1. Results represent mean ± SD from three different experiments (* 𝑃 = . 0 2 9 versus medium).