The Lipid Moiety of Haemozoin (Malaria Pigment) and P. falciparum Parasitised Red Blood Cells Bind Synthetic and Native Endothelin-1

Table 2

Effect of normal RBC, pRBC, or supernatants from P. falciparum cultures on the recovery of synthetic ET-1.

% ET-1 recovery when incubated with:
	Medium	Normal RBC	pRBC (D10)	pRBC (W2)	SN normal RBC	SN pRBC (D10)	SN pRBC (W2)
ET-1^a5	100	91.96 ± 7.2	39.8±8.3^∗	40.2±7.5^∗	102 ± 5.4	98 ± 6.3	96 ± 7.8
+proteaseinhibitor	105.7 ± 2.1	101.7 ± 4.8	29.6±7.3^∗	48.3±11^∗	ND	ND	ND

^aET-1 (5 fmol/mL) was incubated for 24 hours in medium or in the presence of normal unparasitized RBC, pRBC from different P. falciparum strains or supernatants (SNs) from parasite cultures. ET-1 was also incubated with RBC or pRBC in the presence of a protease inhibitor cocktail. At the end of the incubation, ET-1 was detected by ELISA.
Data are expressed as % of ET-1 recovery respect to controls and represent the mean ± SD from three different experiments. ^∗𝑃<.01 versus control.