Table 2: Effect of normal RBC, pRBC, or supernatants from P. falciparum cultures on the recovery of synthetic ET-1.

% ET-1 recovery when incubated with:
MediumNormal RBCpRBC (D10)pRBC (W2)SN normal RBCSN pRBC (D10)SN pRBC (W2)

ET-1a510091.96 ± 7.2 3 9 . 8 ± 8 . 3 4 0 . 2 ± 7 . 5 102 ± 5.498 ± 6.396 ± 7.8
+proteaseinhibitor105.7 ± 2.1101.7 ± 4.8 2 9 . 6 ± 7 . 3 4 8 . 3 ± 1 1 NDNDND

aET-1 (5 fmol/mL) was incubated for 24 hours in medium or in the presence of normal unparasitized RBC, pRBC from different P. falciparum strains or supernatants (SNs) from parasite cultures. ET-1 was also incubated with RBC or pRBC in the presence of a protease inhibitor cocktail. At the end of the incubation, ET-1 was detected by ELISA.
Data are expressed as % of ET-1 recovery respect to controls and represent the mean ± SD from three different experiments. 𝑃 < . 0 1 versus control.