Methodology Report

IMMUNOCAT—A Data Management System for Epitope Mapping Studies

Figure 2

The architecture of IMMUNOCAT system. IMMUNOCAT is an integration of three web accessible relational databases. DONORDB is designed for data collected during donor enrollment and clinical tests. ELICAT stores the data from T cell assays performed using donor blood samples stimulated with a set of allergen derived peptides. TOPCAT stores the MHC-peptide binding data of the same set of peptides. Simplified database structures are presented at the top of the figure. Arrows represent the links between database tables. Tables shared by two databases are colored yellow. In DONORDB, donor information table is linked to skin test, disease state, and MHC allele tables. In ELICAT, table of experimental spot forming cell (SFC) is linked to peptide, T cell source, and MHC allele tables. In TOPCAT, table of experimental IC50 is linked to peptide and MHC allele tables. DONORDB is connected with ELICAT using donor IDs and MHC allele IDs. ELICAT and TOPCAT are connected with each other using shared peptide IDs.
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