Lentiviral-Mediated RNA Interference against TGF-Beta Receptor Type II in Renal Epithelial and Fibroblast Cell Populations In Vitro Demonstrates Regulated Renal Fibrogenesis That Is More
Efficient than a Nonlentiviral Vector
Figure 2
Gene silencing of TBRII by RNAi in HEK-293T cells. pPlasRi-TBRII and pLentiRi-TBRII were transfected into HEK-293T cells. FITC-fluorescence immunohistochemistry of TBRII is demonstrated in the upper two panels. The upper panel demonstrates pPlasRi-TBRII and the lower panel demonstrates pLentiRi-TBRII. Both groups showed a decrease of TBRII expression. TBRII was also evaluated by Western blotting (blot panel) and semiquantified by densitometry (graphical presentation). There was significant inhibition of TBRII after both transfections at all time points (** in comparison with controls), but the lentiviral construct increased with time and caused significantly greater silencing at 72 hrs than pPlasRi-TBRII ().
