Figure 6: mRNA expression of MIG (CXCL9), MCP-1 (CCL2), MIP-1β (CCL4), and Rantes (CCL5) in cardiac tissue of Balb/c mice infected with BT of T. cruzi. Total RNA was obtained at day 21 post infection and chemokines expression was determined by RT-PCR as described in material and methods. PCR products were analyzed in 2% agarose gels stained with ethidium bromide. Semicuantitative analysis was done. Values are average of 4 mice from 3 independent assays. Grey bar control, black bar Ninoa strain and White bar Qro strain. In the case of MIG and MIP-1β no detectable levels of chemokines were found in the control tissues. Significant differences in chemokine expression were found in cardiac tissue of control versus infected mice. Differences between T. cruzi strains were also founded .