Table 1: Comparison of 𝐸 𝑛 𝑡 𝑎 𝑚 𝑜 𝑒 𝑏 𝑎 𝑖 𝑠 𝑡 𝑜 𝑙 𝑦 𝑡 𝑖 𝑐 𝑎 , 𝐻 𝑜 𝑚 𝑜 𝑠 𝑎 𝑝 𝑖 𝑒 𝑛 𝑠 , and 𝑆 𝑎 𝑐 𝑐 𝑎 𝑟 𝑜 𝑚 𝑦 𝑐 𝑒 𝑠 𝑐 𝑒 𝑟 𝑒 𝑣 𝑖 𝑠 𝑖 𝑎 𝑒 ESCRT machineries.

E n t a m o e b a h i s t o l y t i c a H o m o s a p i e n s S a c c h a r o m y c e s c e r e v i s i a e
Putative complexPredicted protein A c c e s i o n n u m b e r a ProteinAccesion n u m b e r b 𝑒 -valueS (%)I (%)ProteinAccesion n u m b e r a 𝑒 -valueS (%)I (%)

ESCRT-0 complexEhHse1EHI_091530STAM1Q92783 9 𝑒 0 8 7250Hse1pP38753 2 𝑒 1 0 7062
EhVps27EHI_117910HRSO14964Vps27pP40343 1 𝑒 1 1 6149

ESCRT-I complexEhVps23EHI_135460TSG101Q99816 1 . 4 𝑒 1 0 5133Vps23pP256040.00523125
EhVps28EHI_108630hVps28Q9UK41Vps28pQ027670.000574528
EhVps37AEHI_077870hVps37AQ6NW270.000225524Vps37pQ991760.000844621
EhVps37DEHI_060400hVps37DQ6P2C3 3 . 8 𝑒 0 6 5035nd
ndndMvb12P42939

ESCRT-II complexEhVps22EHI_131120EAP30Q96H20 9 𝑒 2 2 4730Vps22pQ12483 3 . 4 𝑒 1 5 4825
EhVps25EHI_137860EAP20Q9BRG1 9 𝑒 0 8 5228Vps25pP471420.000384619
EhVps36EHI_045320EAP45Q86VN1 1 . 8 𝑒 1 5 4925Vps36pQ06696 1 . 1 𝑒 0 9 4826

ESCRT-III complexEhVps2EHI_194400CHMP2AO43633 8 . 9 𝑒 2 4 5529Vps2pP36108 2 𝑒 0 5 5029
CHMP2BQ9UQN3 7 . 9 𝑒 1 6 4825
EhVps20EHI_066730CHMP6Q96FZ7Vps20pQ042720.000155426
EhVps24EHI_048690CHMP3Q9Y3E7Vps24pP36095 2 . 2 𝑒 0 5 4822
EhVps32EHI_169820CHMP4AQ9BY430.00124824Vps32pP39929 2 . 5 𝑒 1 2 4825
CHMP4BQ9H444 9 . 6 𝑒 0 7 4320nd
CHMP4CQ96CF2 1 . 4 𝑒 0 6 4320nd

Vps4EhVps4EHI_118900hVps4AQ9UN37 1 . 1 𝑒 1 1 4 6952Vps4pP52917 3 𝑒 1 1 4 7860
hVps4BO75351 3 𝑒 1 1 4 7860

Other MVB proteinsEhVta1EHI_010040hVta1Q9NP79 2 . 8 𝑒 0 5 4423Vta1pQ06263
EhVps46EHI_093850CHMP1AQ9HD42Vps46pP697710.000324818
CHMP1BQ7LBR1nd
EhVps60EHI_114790CHMP5Q9NZZ3 5 . 6 𝑒 1 5 4528Vps60pQ03390 9 𝑒 0 6 4619
EhDoa4EHI_012290UBP4Q13107 1 𝑒 7 7 5836Doa4pP32571 2 𝑒 2 7 5031
EhADH112EHI_181220ALIXQ9UKL5 1 𝑒 2 1 4020Rim20pQ12033 2 𝑒 1 2 3821
BRO1pP485820.0035032

Ubiquitination componentsndndBul1pP48524
EhRsp5EHI_011530NEED4P46934 1 . 6 𝑒 5 0 5333Rsp5pP39940 7 . 8 𝑒 5 7 5736

a P a t h e m a Entamoeba Bioinformatics Resource Center. b S w i s s -Prot/TrEMBL databases. nd: not determined. —: Denote nonsignificant similarity/identity and 𝑒 -values. S: Similarity. I: Identity.