Detection of EBV, HBV, HCV, HIV-1, HTLV-I and -II, and SMRV in Human and Other Primate Cell Lines

Figure 1

PCR analysis of EBV status in cell lines. Shown is an ethidium bromide-stained gel containing the reaction products following PCR amplification with the primers F-EBV and R-EBV (see Table 1). Products of 265 bp were obtained. Shown are various examples of EBV-negative and EBV-positive cell lines. Two paired PCR reactions were performed: one PCR reaction contained an aliquot of the sample only (a) and the second reaction contained the sample under study plus the control DNA as internal standard, which results in an amplified product size of 445 bp (b). The cell cultures EB-1, NAMALWA, and RAJI are EBV-positive; the cell cultures BV-173 and REH, both B-cell precursor leukemia cell lines, are EBV-negative.