Induction by TNF- of IL-6 and IL-8 in Cystic Fibrosis Bronchial IB3-1 Epithelial Cells Encapsulated in Alginate Microbeads
Figure 1
Morphology (a) and viability (insert) of IB3-1 cell monolayers, as determined by bright field and fluorescence photomicrographs. Fluorescence photomicrographs were taken after double staining with Calcein-AM and propidium bromide. Analysis of the release of proinflammatory cytokines by IB3-1 cells, as determinated by Bio-Plex analysis (b,c). The indicated proteins were analyzed in the IB3-1 cell culture medium, namely, IL-1r (Interleukin-1 receptor alpha), G-CSF (Granulocyte-colony stimulating factor), MCP-1 (Monocyte chemotactic protein-1), IL-6 (Interleukin 6), IL-8 (Interleukin 8), RANTES (Regulated upon activation, normal cell expressed and secreted), and VEGF (Vascular endothelial growth factor). Data are referred to control untreated cells (open bars) and to cells treated for 24 hours with TNF- (80 ng/mL) (closed bars). Data represent the average SD ().
