Optimization of Formaldehyde Cross-Linking for Protein Interaction Analysis of Non-Tagged Integrin 1
Figure 3
Analysis of anti-integrin antibodies. (a) Test of antibodies for immunoprecipitation. 0.5 mg Jurkat cell lysate was incubated with 1 g of the indicated antibody and affinity purification was performed. Precipitations were analyzed by JB1A immunoblot (anti-mouse HRP). Two integrin chains were detected in all immunoprecipitations. The heavy (HC) and light chains (LC) of the antibodies are indicated. (b) Amino acid sequence of the extracellular domain of human integrin . Known continuous epitopes are indicated. Underlined amino acids are known to become formaldehyde modified first. (c) Model of the extracellular part of human integrin , based on the crystal structure of Integrin (Swiss Model). Indicated are the same epitopes as in (b).
