A Proteomic Approach for Plasma Biomarker Discovery with iTRAQ Labelling and OFFGEL Fractionation
Table 1
Protein recovery after plasma prefractionation using immunoaffinity depletion of high-abundant proteins or hexapeptide ligand library treatment. Protein content was estimated using FluoroProfile Protein Quantification Kit.
MARS-Hu 14
Proteo Miner
Plasma volume loaded (L)
10 10 depletion cycles
900
Protein quantity loaded (mg) (whole plasma : 63 mg/mL proteins)
6,3
56,7
Protein quantity recovered after prefractionation (g)
Average protein quantity recovered after prefractionation (g)
364 (or 36.4 g per depletion cycle)
1336
% of total protein mass removal by prefractionation
