Research Article
Hypomethylation of IL10 and IL13 Promoters in CD4+ T Cells of Patients with Systemic Lupus Erythematosus
Table 2
Nest-PCR primers for Bisulfite sequencing.
| Gene | Amplication location | Sequence (5’–3’) |
| | round I: forward | | | (+3063 to +3088 bp) | TTGGGGGTTTAGTTTAGATTTTTTAG | IL10 | round I: reverse | | | (+3331 to +3307 bp) | TTCACACCCCCTAATATTAACACTC | | round II: forward | | | (+3119 to +3143 bp) | ATTTAATTTGGAGTTGGTTTAAGTT | | round II: reverse | | | (+3310 to +3285 bp) | ACTCTATACAATATCTATCCCCAACC |
| | round I: forward | | | (−2425 to −2401 bp) | TTGATTGGGTATTATGTTTTTGTTA | | round I: reverse | | | (−1537 to −1561 bp) | AACTACCTTAATCTATACAACTCCC | IL13 | round II: forward | | | (−2334 to −2312 bp) | GAGGAAGGGAGGTTTTAATTTTA | | round II: reverse | | | (−2045 to −2069 bp) | ACCCTAAAAAATAATCTCCAAAAAC |
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