Hypomethylation of IL10 and IL13 Promoters in CD4⁺ T Cells of Patients with Systemic Lupus Erythematosus

Table 2

Nest-PCR primers for Bisulfite sequencing.

Gene	Amplication location	Sequence (5^’–3^’)
	round I: forward
	(+3063 to +3088 bp)	TTGGGGGTTTAGTTTAGATTTTTTAG
IL10	round I: reverse
	(+3331 to +3307 bp)	TTCACACCCCCTAATATTAACACTC
	round II: forward
	(+3119 to +3143 bp)	ATTTAATTTGGAGTTGGTTTAAGTT
	round II: reverse
	(+3310 to +3285 bp)	ACTCTATACAATATCTATCCCCAACC
	round I: forward
	(−2425 to −2401 bp)	TTGATTGGGTATTATGTTTTTGTTA
	round I: reverse
	(−1537 to −1561 bp)	AACTACCTTAATCTATACAACTCCC
IL13	round II: forward
	(−2334 to −2312 bp)	GAGGAAGGGAGGTTTTAATTTTA
	round II: reverse
	(−2045 to −2069 bp)	ACCCTAAAAAATAATCTCCAAAAAC