Mass Spectrometric Analysis of Multiple Pertussis Toxins and Toxoids
Figure 1
MS/MS spectra illustrating similar fragmentation profiles of Subunit 1 peptide GTGHFIGIYEVR from representative pertussis toxin (B) and pertussis toxoid (C). Nano Liquid Chromatography MS/MS were performed using the LTQ-Orbitrap on tryptic peptides obtained from pertussis toxin B and pertussis toxoid C. The MS/MS data was searched against the NCBI database and the peptide spectrum was analyzed [comparing relative peak intensity (0–100%) versus mass/charge (0 to 1500)] using the Scaffold software. The red and blue lines represent y- and b-ion distribution peaks, respectively.
