Mass Spectrometric Analysis of Multiple Pertussis Toxins and Toxoids

Table 1

Comparative toxin and toxoid sequence analysis (% amino acid coverage).

	A-1	A-2	A-3	B-1	B-2	B-3	C-1	C-2	C-3	D-1	D-2	D-3
S1	30	58	26	35	69	55	36	49	61	23	41	58
S2	6	33	28	20	39	31	ND	14	16	ND	5	19
S3	ND	28	18	18	39	49	ND	12	22	ND	18	20
S4	20	53	13	41	57	42	ND	28	ND	ND	28	ND
S5	ND	ND	8	ND	ND	8	ND	ND	ND	ND	ND	8

-ND: Not Detected
-Pertussis toxin Subunits (S1–S5)
-Sources: Pertussis toxins (A and B) and Pertussis toxoids (C and D)
-Instruments: 1–MALDI-TOF MS (4800 Proteomics Analyzer), 2–ESI-MS (LTQ-Orbitrap), and 3–ESI-MS (Q-Tof)