Research Article
Mass Spectrometric Analysis of Multiple Pertussis Toxins and Toxoids
Table 4
Peptide detection among pertussis toxin and toxoid subunits (/+ rapigest treatment).
| | RG | + RG |
| | S1 | | GTGHFIGYIYEVR (114–126) | | GTGHFIGYIYEVR (114–126) | YTEVYLEHR (93–101) | | YTEVYLEHR (93–101) | MQEAVEAER (102–110) | | MQEAVEAER (102–110) | IPPENIR (170–176) | | IPPENIR (170–176) | VYHNGITGETTTTEYSNAR (181–199) | | ILAGALATYQSEYLAHR (152–168) | | MAPVIGACMAR (228–238) |
| | S2 | GSGDLQEYLR (64–73) | GSGDLQEYLR (64–73) | | NTGQPATDHYYSNVTATR (120–137) |
| | S3 | ALFTQQGGAYGR (39–50) | ALFTQQGGAYGR (39–50) | | QITPGWSIYGLYDGTYLGQAYGGIIK (75–100) | | GNAELQTYLR (65–74) | | DAPPGAGFIYR (101–111) |
| | S4 | TNMVVTSVAMKPYEVTPTR (51–69) | TNMVVTSVAMKPYEVTPTR (51–69) | | RPGSSPMEVMLR (100–111) |
| | S5 | | NFTVQELALK (43–52) |
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-Peptides detected in +RG (rapigest) (in bold) -Approach: Electrospray Ionization Mass Spectrometry-Instrument: LTQ-Orbitrap only
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