Research Article

Proteomic Analysis of Pichindé virus Infection Identifies Differential Expression of Prothymosin-

Figure 5

(a) Induction of (pro)thymosin- 𝛼 expression by PICV infection. P388D1 cells were infected with either P2 or P18 PICV in triplicate and tissue culture supernatants collected at various times post infection for determination of (pro)thymosin- 𝛼 levels by ELISA. Fold increases are compared to levels from triplicate samples at time = 0 and show the results from three experiments. Error bars show the standard error of the mean, and asterisks indicate significant ( 𝑃 < . 0 5 ) differences in levels induced between P2 and P18 infection as determined by Student’s 𝑡 -test. (b) Changes in stathmin expression following infection by P2 and P18 PICV. Triplicate cultures of P388D1 cells were infected with PICV and whole cell lysates assayed or stathmin levels by immunoblotting.
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