BioMed Research International

Research Article

Expression and Purification of Z Protein from Junín Virus

Table 1

Biochemical properties of the different expressed proteins.
ProteinNumber of amino acidsMW (Da)pIExtinction coefficients1
𝑍 10611560.18.44 1 5 9 7 0 / 1 5 4 7 0
Tio-Z-V5-His25127339.16.29 3 0 0 7 5 / 2 9 4 5 0
Tio12012814.54.71 1 4 1 0 5 / 1 3 9 8 0
Z-V5-His13114542.58.43 1 5 9 7 0 / 1 5 4 7 0
GST-Z33137855.66.79 5 9 0 8 0 / 5 8 3 3 0
GST22526293.26.00 4 3 1 1 0 / 4 2 8 6 0
His-Z13615432.47.09 2 1 9 3 0 / 2 1 4 3 0
1Calculated extinction coefficients in M − 1 cm-1 units, measured to 280 nm in water. Left numbers correspond to the calculation assuming that all Cys residues appear as half cystines, while the right numbers correspond to the calculation assuming that no Cys residues appear as half cystines.