Expression and Purification of Z Protein from Junín Virus

Table 2

Quantification data for expression and purification of GST-Z and Tio-Z-V5-His recombinants proteins.

GST-Z			Tio-Z-V5-His
Step	Relative Z-GST(%)^1	Purification factor	Step	Relative Tio-Z (%)^1	Purification factor
Total lysate	21	0.0	Total lysate	19	0.0
GST-affinity^2	88	4.1	Tio- affinity	63	3.3
Gel filtration^2	91	4.2	Gel filtration	94	4.9

¹Soluble protein ratio (Z fussion protein/total protein). ² Include degradation products of Z.