Novel Method of Cell-Free In Vitro Synthesis of the Human Fibroblast Growth Factor 1 Gene

Table 1

List of oligonucleotides.

Oligonucleotide	Sequence (5^ to 3^)
pcFGF1F	gtccagtgtggtggaattgcccttaccatggctgaaggggaaatcaccacc
pcFGF1R12-71	TTG TAA TTC CCT GGA GGC AGA TTA AAC TTC TCG GTC AGG
	GCT GTG AAG GTG GTG ATT TCC
pcFGF1F42-101	gaagtttaatctgcctccagggaattacaagaagcccaaactcctctactgtagcaacgg
pcFGF1R76-135	TGT GCC ATC CGG AAG GAT CCT CAG GAA GTG GCC CCC
	GTT GCTACAGTAGAGGAGTTTGGG
pcFGF1F114-173	gaggatccttccggatggcacagtggatgggacaagggacaggagcgaccagcacattca
pcFGF1R151-210	ATA CAC CTC CCC CAC GCT TTC CGC ACT GAG CTG CAG CTG
	AAT GTGCTGGTCGCTCCTGTC
pcFGF1F188-247	cggaaagcgtgggggaggtgtatataaagagtaccgagactggccagtacttggccatgg
pcFGF1R225-284	ACA TTC CTC ATT TGG TGT CTG TGA GCC GTA TAA AAG CCC
	GTC GGTGTCCATGGCCAAGTACTGGCCAGTC
pcFGF1F268-327	ggctcacagacaccaaatgaggaatgtttgttcctggaaaggctggaggagaaccattac
pcFGF1R301-360	CTT CTC TGC ATG CTT CTT GGA TAT ATA GGT GTT GTA ATG
	GTT CTC CTCCAGCCTTTCCAG
pcFGF1F330-389	cacctatatatccaagaagcatgcagagaagaattggtttgttggcctcaagaagaatgg
pcFGF1R365-424	GGC CAT AGT GAG TCC GAG GAC CGC GTT TGC AGC TCC
	CAT TCT TCTTGAGGCCAACAAACC
pcFGF1F402-461	cggtcctcggactcactatggccagaaagcaatcttgtttctccccctgccagtctcttc
pcFGF1R	GCTGGATATCTGCAGAATTGCCCTT TTA ATC AGA AGA
	GACTGGCAGGGGG