Constitutive High Level Expression of an Endoxylanase Gene from the Newly Isolated Bacillus subtilis AQ1 in Escherichia coli

Table 1

Physiological and biochemical characteristics of isolates AQ1 based on the API 50 CHB system and other tests described in Materials and Methods. (+) presence; (−) absence.

Properties detected	Isolate AQ1
Colony color	White
Colony shape	Spread
Elevation	Flat
Gram staining	+
Endospore	Central
Growth at 50°C	+
Growth at 7% NaCl containing medium	+
Growth in anaerobic agar	+
Cell shape	Rod
Hydrolysis of indole	−
Methyl Red	−
Voges-Proskauer	+
Citrate	+
Catalase	+
Nitrate reduction	+
Slime formation	+
Carbohydrates metabolism:
Glycerol	+
Erythritol	−
D-arabinose	−
L-arabinose	+
Ribose	+
D-xylose	+
L-xylose	−
Adonitol	−
beta-methyl-D-xyloside	−
Galactose	+
Glucose	+
Fructose	+
Mannose	+
Sorbose	−
Rhamnose	−
Dulcitol	−
Galactose	+
Glucose	+
Fructose	+
Mannose	+
Sorbose	−
Rhamnose	−
Dulcitol	−
Inositol	+
Mannitol	+
Sorbitol	+
Alpha-methyl-D-mannoside	−
Alpha-methyl-D-glucoside	+
N-acetyl-glucosamine	−
Amygdalin	+
Arbutin	+
Esculin	+
Salicin	+
Cellobiose	+
Maltose	+
Lactose	+
Melibiose	+
Sucrose	+
Trehalose	+
Inulin	−
Melezitose	−
Raffinose	+
Starch	+
Glycogen	+
Xylitol	−
Gentiobiose	+
D-turanose	−
D-lyxose	−
D-tagatose	−
D-fucose	−
D-arabitol	−
D-arabitol	−
L-arabitol	−
Gluconate	−
2-keto-gluconate	−
5-keto-gluconate	−