132975.fig.002
Figure 2: An example of two-color BAC-FISH showing hybridizations of BAC clones containing sex-linked genes from snake and chicken in a metaphase chromosome spread from an Australian bearded dragon (Pogona vitticeps). Red arrows indicate hybridization signals of a BAC clone containing chicken sex-linked gene CHD1 (red signals) onto the short arms of chromosomes 2 in P. vitticeps; green arrows indicate hybridization signals of a BAC clone containing snake sex-linked gene KLF6 onto the short arms of chromosomes 6 in P. vitticeps, thus showing nonhomology of ZZ/ZW sex chromosomes in reptiles [5]. Scale bar indicates 10 m.