The Effects of Fractions from Shiitake Mushroom on Composition and Cariogenicity of Dental Plaque Microcosms in an In Vitro Caries Model
Table 1
Summary of the mineral loss data (IML—integrated mineral loss, and LD—lesion depth) from the dentin specimens ( per treatment or control) that were used as substrata for biofilm growth in CDFF for 8 days; amount of protein (protein) and amount of main acid anions (acetate, lactate, propionate, and succinate) in the resting state (Resting) and after 30 min incubation with 1% glucose (Fermenting) of the microcosm biofilms ( per treatment or control). The data are average and standard deviations (SD) from samples obtained from 2 CDFF runs per treatment. CHX—chlorhexidine digluconate; MLMW—low molecular weight fraction of mushroom; SF4—subfraction nr 4 of MLMW fraction; SF5—subfraction nr 5 of MLMW fraction.
Negative control (water) and the respective treatments
IML vol% × μm (SD)
LD μm (SD)
Protein μg (SD)
Acetate nmol/sample (SD)
Lactate nmol/sample (SD)
Propionate nmol/sample (SD)
Succinate nmol/sample (SD)
Resting
Fermenting
Resting
Fermenting
Resting
Fermenting
Resting
Fermenting
Water (CHX)
2948
122
104
1051
947
674
1878
198
457
905
718
(1072)
(29)
(44)
(294)
(163)
(205)
(504)
(85)
(240)
(327)
(166)
CHX
508*
43*
53*
728*
789
752
1021*
28*
27*
809
1021*
(263)
(20)
(30)
(385)
(427)
(395)
(387)
(86)
(87)
(399)
(373)
Water (MLMW)
1696
83
179
940
1273
595
1986
168
506
658
826
(678)
(24)
(60)
(451)
(372)
(183)
(462)
(111)
(190)
(227)
(277)
MLMW
1717
87
194
1488*
1102
851*
1940
418*
469
805
639
(842)
(29)
(172)
(436)
(321)
(248)
(684)
(144)
(239)
(103)
(99)
Water (SF4)
4293
168
135
1964
1710
877
1307
117
324
1254
1292
(2536)
(69)
(44)
(492)
(157)
(139)
(552)
(134)
(172)
(235)
(166)
SF4
802*
57*
123
1667
2189
745*
1908*
167
176
1214
1106*
(302)
(15)
(62)
(145)
(949)
(125)
(316)
(145)
(178)
(217)
(176)
Water (SF5)
1918
113
197
2033
2733
623
1719
7.5
407
739
1586
(761)
(36)
(130)
(500)
(1438)
(182)
(800)
(18)
(295)
(142)
(967)
SF5
1537
88*
124
2923*
1958
1261
1435
75
121*
1142
907*
(317)
(15)
(40)
(1917)
(1115)
(686)
(364)
(118)
(131)
(720)
(145)
*Statistically significantly different from the respective water control samples (independent samples t-test,).
