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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 157458, 7 pages
http://dx.doi.org/10.1155/2011/157458
Research Article

Novel Concept of Motor Functional Analysis for Spinal Cord Injury in Adult Mice

1Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo 160-8582, Japan
2Department of Neurosurgery, Graduate School of Medicine, University of Tokyo, Tokyo 113-8655, Japan
3Department of Orthopedic Surgery, Keio University School of Medicine, Shinjuku, Tokyo 160-8582, Japan
4Department of Rehabilitation Medicine, Keio University School of Medicine, Shinjuku, Tokyo 160-8582, Japan

Received 9 September 2010; Accepted 2 December 2010

Academic Editor: Monica Fedele

Copyright © 2011 Munehisa Shinozaki et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In basic research on spinal cord injury (SCI), behavioral evaluation of the SCI animal model is critical. However, it is difficult to accurately evaluate function in the mouse SCI model due to the small size of mice. Although the open-field scoring scale is an outstanding appraisal method, supplementary objective tests are required. Using a compact SCANET system, in which a mouse carries out free movement for 5 min, we developed a novel method to detect locomotor ability. A SCANET system samples the horizontal coordinates of a mouse every 0.1 s, and both the speed and acceleration of its motion are calculated at each moment. It was found that the maximum speed and acceleration of motion over 5 min varied by injury severity. Moreover, these values were significantly correlated with open-field scores. The maximum speed and acceleration of SCI model mice using a SCANET system are objective, easy to obtain, and reproducible for evaluating locomotive function.