Bioactivity Determination of Native and Variant Forms of Therapeutic Interferons
Figure 1
HEK293-ISRE-Luc cell line responses to treatment with various types of interferons. Panel (a) represents dose response curve obtained with IFN-β. Panel (b) represents dose response curve obtained with IFN-α. The assay was performed as described in “Section 2”. The cells were treated with type I IFN, followed by the analyses of luciferase activity. As expected, although IFN-α and IFN-β are both type I IFNs that drive the ISRE, the signalling pathway used is slightly different and thus we obtain a different dose response curve.
