Table 1: Matrix interference.

LL of estimated potencyEstimated potencyUL estimated PotencyLL of relative potency  (%)Relative potencyUL of relative potency

IL-20.9624611.010241.0604596.2101.00106.00
IL-60.9292880.9755311.0239392.997.60102.40
IL-1β0.9441260.9910461.0402494.499.10104.00
TNFα1.07841.132081.18928107.8113.20118.90

The table shows data from one concentration of IL-2, IL-6, IL-1β, or TNF-α. Wider concentrations of IL-2, IL-6, IL-1β and TNF-α found no interference with our assay (not shown in the table). The tested ranges are the following: IL-2: 200–2000 IU/mL; IL-6: 200–2000 IU/mL; IL-1β: 40–400 U/mL; TNF-α: 100–1000 ng/mL. The values in bold face represent relative potency compared with the reference standards as determined by statistical analyses. LL stands for lower limits of the 95% interval while UL denotes upper limits of the 95% interval.