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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 185683, 8 pages
http://dx.doi.org/10.1155/2011/185683
Research Article

Cardiac Imaging Using Clinical 1.5 T MRI Scanners in a Murine Ischemia/Reperfusion Model

1Department of Internal Medicine III (Cardiology), Innsbruck Medical University, Anichstr. 35, 6020 Innsbruck, Austria
2Department of Radiology I, Innsbruck Medical University, Anichstr. 35, 6020 Innsbruck, Austria

Received 13 July 2010; Accepted 26 October 2010

Academic Editor: Andrea Vecchione

Copyright © 2011 Jakob G. J. Voelkl et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

To perform cardiac imaging in mice without having to invest in expensive dedicated equipment, we adapted a clinical 1.5 Tesla (T) magnetic resonance imaging (MRI) scanner for use in a murine ischemia/reperfusion model. Phase-sensitive inversion recovery (PSIR) sequence facilitated the determination of infarct sizes in vivo by late gadolinium enhancement. Results were compared to histological infarct areas in mice after ischemia/reperfusion procedure with a good correlation ( π‘Ÿ = 0 . 8 0 7 , 𝑃 < . 0 0 1 ). In addition, fractional area change (FAC) was assessed with single slice cine MRI and was matched to infarct size ( π‘Ÿ = βˆ’ 0 . 8 3 7 ) and fractional shortening (FS) measured with echocardiography ( π‘Ÿ = 0 . 8 6 0 ); both 𝑃 < . 0 0 1 . Here, we demonstrate the use of clinical 1.5 MRI scanners as a feasible method for basic phenotyping in mice. These widely available scanners are capable of investigating in vivo infarct dimensions as well as assessment of cardiac functional parameters in mice with reasonable throughput.