Separation and Identification of HSP-Associated Protein Complexes from Pancreatic Cancer Cell Lines Using 2D CN/SDS-PAGE Coupled with Mass Spectrometry
Figure 1
6% (1), 5–10% (2), and 5–12% (3) separating gels were used to separate the protein complexes from SW1990 cell lysate. M: GE Healthcare HMW-Native protein markers; S: cell lysate of SW1990 (a). SDS-PAGE of ten protein complexes labeled with A–J from 5–12% CN-PAGE. At least two-band combination of each complex was used to run SDS-PAGE (b).
