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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 212819, 7 pages
http://dx.doi.org/10.1155/2011/212819
Research Article

Explanting Is an Ex Vivo Model of Renal Epithelial-Mesenchymal Transition

1Department of Nephrology, The Royal Melbourne Hospital, Melbourne, VIC 3050, Australia
2School of Medical Sciences, RMIT University, Melbourne, VIC 3083, Australia
3Department of Medicine, The University of Melbourne, Melbourne, VIC 3010, Australia

Received 23 June 2011; Revised 1 September 2011; Accepted 1 September 2011

Academic Editor: Nick Di Girolamo

Copyright © 2011 Catherine E. Winbanks et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. A. A. Eddy, “Progression in chronic kidney disease,” Advances in Chronic Kidney Disease, vol. 12, no. 4, pp. 353–365, 2005. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  2. T. D. Hewitson, “Renal tubulointerstitial fibrosis: common but never simple,” American Journal of Physiology, vol. 296, no. 6, pp. F1239–F1244, 2009. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  3. I. Darby, O. Skalli, and G. Gabbiani, “α-Smooth muscle actin is transiently expressed by myofibroblasts during experimental wound healing,” Laboratory Investigation, vol. 63, no. 1, pp. 21–29, 1990. View at Scopus
  4. T. D. Hewitson and G. J. Becker, “Interstitial myofibroblasts in IgA glomerulonephritis,” American Journal of Nephrology, vol. 15, no. 2, pp. 111–117, 1995. View at Scopus
  5. M. Iwano, A. Fischer, H. Okada et al., “Conditional abatement of tissue fibrosis using nucleoside analogs to selectively corrupt DNA replication in transgenic fibroblasts,” Molecular Therapy, vol. 3, no. 2, pp. 149–159, 2001. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  6. Y. Liu, “New insights into epithelial-mesenchymal transition in kidney fibrosis,” Journal of the American Society of Nephrology, vol. 21, no. 2, pp. 212–222, 2010. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  7. M. Iwano, D. Plieth, T. M. Danoff, C. Xue, H. Okada, and E. G. Neilson, “Evidence that fibroblasts derive from epithelium during tissue fibrosis,” Journal of Clinical Investigation, vol. 110, no. 3, pp. 341–350, 2002. View at Publisher · View at Google Scholar · View at Scopus
  8. K. Jinde, D. J. Nikolic-Paterson, X. R. Huang et al., “Tubular phenotypic change in progressive tubulointerstitial fibrosis in human glomerulonephritis,” American Journal of Kidney Diseases, vol. 38, no. 4, pp. 761–769, 2001. View at Scopus
  9. Y. Y. Ng, T. P. Huang, W. C. Yang et al., “Tubular epithelial-myofibroblast transdifferentiation in progressive tubulointerstitial fibrosis in 5/6 nephrectomized rats,” Kidney International, vol. 54, no. 3, pp. 864–876, 1998. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  10. S. E. Quaggin and A. Kapus, “Scar wars: mapping the fate of epithelial-mesenchymal-myofibroblast transition,” Kidney International, vol. 80, no. 1, pp. 41–50, 2011. View at Publisher · View at Google Scholar · View at PubMed
  11. M. Zeisberg and J. S. Duffield, “Resolved: EMT produces fibroblasts in the kidney,” Journal of the American Society of Nephrology, vol. 21, no. 8, pp. 1247–1253, 2010. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  12. B. D. Humphreys, S. L. Lin, A. Kobayashi et al., “Fate tracing reveals the pericyte and not epithelial origin of myofibroblasts in kidney fibrosis,” American Journal of Pathology, vol. 176, no. 1, pp. 85–97, 2010. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  13. J. P. Thiery and J. P. Sleeman, “Complex networks orchestrate epithelial-mesenchymal transitions,” Nature Reviews Molecular Cell Biology, vol. 7, no. 2, pp. 131–142, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  14. G. Prindull and D. Zipori, “Environmental guidance of normal and tumor cell plasticity: epithelial mesenchymal transitions as a paradigm,” Blood, vol. 103, no. 8, pp. 2892–2899, 2004. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  15. T. D. Hewitson, M. G. Tait, K. J. Kelynack, M. Martic, and G. J. Becker, “Dipyridamole inhibits in vitro renal fibroblast proliferation and collagen synthesis,” Journal of Laboratory and Clinical Medicine, vol. 140, no. 3, pp. 199–208, 2002. View at Publisher · View at Google Scholar · View at Scopus
  16. T. D. Hewitson, K. J. Kelynack, M. G. Tait et al., “Pirfenidone reduces in vitro rat renal fibroblast activation and mitogenesis,” Journal of Nephrology, vol. 14, no. 6, pp. 453–460, 2001. View at Scopus
  17. F. G. Silva, T. Nadasdy, and Z. Laszik, “Immunohistochemical and lectin dissection of the human nephron in health and disease,” Archives of Pathology and Laboratory Medicine, vol. 117, no. 12, pp. 1233–1239, 1993. View at Scopus
  18. L. D. Truong, V. T. Phung, Y. Yoshikawa, and C. A. Mattioli, “Glycoconjugates in normal human kidney. A histochemical study using 13 biotinylated lectins,” Histochemistry, vol. 90, no. 1, pp. 51–60, 1988. View at Scopus
  19. S. E. Wong, C. E. Winbanks, C. S. Samuel, and T. D. Hewitson, “Lectin histochemistry for light and electron microscopy,” Methods in Molecular Biology, vol. 611, pp. 103–114, 2010. View at Scopus
  20. J. M. Lee, S. Dedhar, R. Kalluri, and E. W. Thompson, “The epithelial-mesenchymal transition: new insights in signaling, development, and disease,” Journal of Cell Biology, vol. 172, no. 7, pp. 973–981, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  21. Y. Khew-Goodall and C. Wadham, “A perspective on regulation of cell-cell adhesion and epithelial- mesenchymal transition: known and novel,” Cells Tissues Organs, vol. 179, no. 1-2, pp. 81–86, 2005. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  22. I. R. Gupta, M. Lapointe, and O. H. Yu, “Morphogenesis during mouse embryonic kidney explant culture,” Kidney International, vol. 63, no. 1, pp. 365–376, 2003. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  23. B. Boyer, G. C. Tucker, A. M. Valles, W. W. Franke, and J. P. Thiery, “Rearrangements of desmosomal and cytoskeletal proteins during the transition from epithelial to fibroblastoid organization in cultured rat bladder carcinoma cells,” Journal of Cell Biology, vol. 109, no. 4, pp. 1495–1509, 1989. View at Scopus
  24. B. Hinz, S. H. Phan, V. J. Thannickal, A. Galli, M. L. Bochaton-Piallat, and G. Gabbiani, “The myofibroblast: one function, multiple origins,” American Journal of Pathology, vol. 170, no. 6, pp. 1807–1816, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  25. J. Yang and Y. Liu, “Dissection of key events in tubular epithelial to myofibroblast transition and its implications in renal interstitial fibrosis,” American Journal of Pathology, vol. 159, no. 4, pp. 1465–1475, 2001. View at Scopus
  26. D. J. Dwivedi, G. Pino, A. Banh et al., “Matrix metalloproteinase inhibitors suppress transforming growth factor-β-induced subcapsular cataract formation,” American Journal of Pathology, vol. 168, no. 1, pp. 69–79, 2006. View at Publisher · View at Google Scholar · View at Scopus
  27. A. Masszi, L. Fan, L. Rosivall et al., “Integrity of cell-cell contacts is a critical regulator of TGF-β1-induced epithelial-to-myofibroblast transition: role for β-catenin,” American Journal of Pathology, vol. 165, no. 6, pp. 1955–1967, 2004. View at Scopus
  28. A. Masszi, P. Speight, E. Charbonney et al., “Fate-determining mechanisms in epithelial-myofibroblast transition: major inhibitory role for Smad3,” Journal of Cell Biology, vol. 188, no. 3, pp. 383–399, 2010. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  29. P. C. Baer, U. W. Tunn, G. Nunez, J. E. Scherberich, and H. Geiger, “Transdifferentiation of distal but not proximal tubular epithelial cells from human kidney in culture,” Experimental Nephrology, vol. 7, no. 4, pp. 306–313, 1999. View at Scopus
  30. M. D. Oldfield, L. A. Bach, J. M. Forbes et al., “Advanced glycation end products cause epithelial-myofibroblast transdifferentiation via the receptor for advanced glycation end products (RAGE),” Journal of Clinical Investigation, vol. 108, no. 12, pp. 1853–1863, 2001. View at Publisher · View at Google Scholar · View at Scopus
  31. J. W. G. V. Copeland, B. W. Beaumont, M. J. Merrilees, and H. L. Pilmore, “Epithelial-to-mesenchymal transition of human proximal tubular epithelial cells: effects of rapamycin, mycophenolate, cyclosporin, azathioprine, and methylprednisolone,” Transplantation, vol. 83, no. 6, pp. 809–814, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  32. M. J. Butt, A. F. Tarantal, D. F. Jimenez, and D. G. Matsell, “Collecting duct epithelial-mesenchymal transition in fetal urinary tract obstruction,” Kidney International, vol. 72, no. 8, pp. 936–944, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus