The Utilization of Triton X-100 for Enhanced Two-Dimensional Liquid-Phase Proteomics
Figure 3
PF2D results of Beckman Coulter’s protein test mixture containing five proteins (Ribonuclease A, insulin, α-lactalbumin, trypsin inhibitor, cholecystokinin (CCK)). (A) First-dimension elution profile of five proteins generated using 0.15% Triton X-100 over 200 min. The elution profiles were monitored at 280 nm. (B) ProteoVue 2D map of five proteins having pI 8.15–4.00 eluted in the order of increasing hydrophobicity. The fraction profiles were monitored at 214 nm.
