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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 219060, 11 pages
http://dx.doi.org/10.1155/2011/219060
Research Article

Investigation of Hepatoprotective Activity of Induced Pluripotent Stem Cells in the Mouse Model of Liver Injury

1Institute of Pharmacology, National Yang-Ming University, No. 155, Section 2, Linong Street, Taipei 11221, Taiwan
2Division of Urology, Department of Surgery, Taipei Veterans General Hospital and Su-Ao & Yuan-Shan Branch, No. 201, Section 2, Shih-Pai Road, Taipei 11217, Taiwan
3School of Medicine, National Yang-Ming University, No. 155, Section 2, Linong Street, Taipei 11221, Taiwan
4Division of General Medicine, Taipei Veterans General Hospital, No. 201, Section 2, Shih-Pai Road, Taipei 11217, Taiwan
5Division of Gastroenterology, Taipei Veterans General Hospital, No. 201, Section 2, Shih-Pai Road, Taipei 11217, Taiwan
6Department of Obstetrics and Gynecology, Taipei Veterans General Hospital, No. 201, Section 2, Shih-Pai Road, Taipei 11217, Taiwan
7Graduate Institute of Pharmacy, National Defense Medical Center, No. 161, Section 6, Minquan E. Road, Taipei 11490, Taiwan
8Department of Medical Research & Education, Taipei Veterans General Hospital, No. 201, Section 2, Shih-Pai Road, Taipei 11217, Taiwan

Received 5 April 2011; Accepted 27 May 2011

Academic Editor: Ken-ichi Isobe

Copyright © 2011 Chih-Hung Chiang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

To date liver transplantation is the only effective treatment for end-stage liver diseases. Considering the potential of pluripotency and differentiation into tridermal lineages, induced pluripotent stem cells (iPSCs) may serve as an alternative of cell-based therapy. Herein, we investigated the effect of iPSC transplantation on thioacetamide- (TAA-) induced acute/fulminant hepatic failure (AHF) in mice. Firstly, we demonstrated that iPSCs had the capacity to differentiate into hepatocyte-like cells (iPSC-Heps) that expressed various hepatic markers, including albumin, α-fetoprotein, and hepatocyte nuclear factor-3β, and exhibited biological functions. Intravenous transplantation of iPSCs effectively reduced the hepatic necrotic area, improved liver functions and motor activity, and rescued TAA-treated mice from lethal AHF. 1 , 1 -dioctadecyl-3,3, 3 , 3 -tetramethylindocarbocyanine perchlorate cell labeling revealed that iPSCs potentially mobilized to the damaged liver area. Taken together, iPSCs can effectively rescue experimental AHF and represent a potentially favorable cell source of cell-based therapy.